CalDAG-GEFI Antibody / RASGRP2

The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain, 2 tandem repeats of EF-hand calcium-binding motifs, and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases, including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Three alternatively spliced transcript variants encoding the same protein have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:25, Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000

UniProt

Q7LDG7

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 7-34 from the human protein was used as the immunogen for the CalDAG-GEFI antibody.

Clonality

Polyclonal

Isotype

Applications

IF, IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the CalDAG-GEFI antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CalDAG-GEFI antibody is available for research use only.

Storage Conditions

Aliquot the CalDAG-GEFI antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the CalDAG-GEFI antibody may be required due to differences in protocols and secondary/substrate sensitivity.