KAP1 Antibody / TRIM28

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:25, Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000

UniProt

Q13263

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 178-207 from the human protein was used as the immunogen for the KAP1 antibody.

Clonality

Polyclonal

Isotype

Applications

IF, IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the KAP1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This KAP1 antibody is available for research use only.

Storage Conditions

Aliquot the KAP1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the KAP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear

Image Legend

Western blot testing of human 1) HeLa and 2) PC-3 cell lysate with KAP1 antibody. Expectd molecular weight: 88~110 kDa depending on sumoylation level.