COL8A2 Antibody

This gene encodes the alpha 2 chain of type VIII collagen. The protein is a major component of the basement membrane of the corneal endothelium and forms homo- or heterotrimers with alpha 1 (VIII) type collagens. Defects in this gene are associated with Fuchs endothelial corneal dystrophy and posterior polymorphous corneal dystrophy type 2.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:25, Immunofluorescence: 1:25, Western blot: 1:500-1:2000

UniProt

P25067

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 571-599 from the human protein was used as the immunogen for the COL8A2 antibody.

Clonality

Polyclonal

Isotype

Applications

IHC-P, IF, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the COL8A2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This COL8A2 antibody is available for research use only.

Storage Conditions

Aliquot the COL8A2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the COL8A2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of 1) human SH-SY5Y, 2) human Jurkat, 3) mouse Neuro-2a and 4) mouse C2C12 cell lysate with COL8A2 antibody. Predicted molecular weight ~67 kDa.