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TRIM65 Antibody

TRIM65 belongs to the TRIM/RBCC family. It contains one B box-type zinc finger, one B30.2/SPRY domain and one RING-type zinc finger.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25, Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000

UniProt

Q6PJ69

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 327-355 from the human protein was used as the immunogen for the TRIM65 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

FACS, IF, IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the TRIM65 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This TRIM65 antibody is available for research use only.

Storage Conditions

Aliquot the TRIM65 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the TRIM65 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, nuclear

Image Legend

IHC testing of FFPE human prostate carcinoma tissue with TRIM65 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

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