BNIP3L Antibody / Nix

This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. The protein encoded by this gene is a functional homolog of BNIP3, a proapoptotic protein. This protein may function simultaneously with BNIP3 and may play a role in tumor suppression.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25, Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000

UniProt

O60238

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 52-81 from the human protein was used as the immunogen for the BNIP3L antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, IF, IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the BNIP3L antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This BNIP3L antibody is available for research use only.

Storage Conditions

Aliquot the BNIP3L antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the BNIP3L antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, nuclear

Image Legend

IHC testing of FFPE human uterus tissue with BNIP3L antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.