Slingshot homolog 3 Antibody / SSH3

The ADF (actin-depolymerizing factor) /cofilin family is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1) . The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000

UniProt

Q8TE77

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 575-602 from the human protein was used as the immunogen for the Slingshot homolog 3 antibody.

Clonality

Polyclonal

Isotype

Applications

IF, FACS, IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Slingshot homolog 3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Slingshot homolog 3 antibody is available for research use only.

Storage Conditions

Aliquot the Slingshot homolog 3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Slingshot homolog 3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, nuclear

Image Legend

IHC testing of FFPE human breast carcinoma tissue with Slingshot homolog 3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.