GALNS Antibody

This gene encodes N-acetylgalactosamine-6-sulfatase which is a lysosomal exohydrolase required for the degradation of the glycosaminoglycans, keratan sulfate, and chondroitin 6-sulfate. Sequence alterations including point, missense and nonsense mutations, as well as those that affect splicing, result in a deficiency of this enzyme. Deficiencies of this enzyme lead to Morquio A syndrome, a lysosomal storage disorder. [provided by RefSeq].

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25, Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25

UniProt

P34059

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 236-263 from the human protein was used as the immunogen for the GALNS antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, IF, WB, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the GALNS antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This GALNS antibody is available for research use only.

Storage Conditions

Aliquot the GALNS antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the GALNS antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human stomach tissue with GALNS antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.