Retinal guanylyl cyclase 1 Antibody / GUCY2D

This gene encodes a retina-specific guanylate cyclase, which is a member of the membrane guanylyl cyclase family. Like other membrane guanylyl cyclases, this enzyme has a hydrophobic amino-terminal signal sequence followed by a large extracellular domain, a single membrane spanning domain, a kinase homology domain, and a guanylyl cyclase catalytic domain. In contrast to other membrane guanylyl cyclases, this enzyme is not activated by natriuretic peptides. Mutations in this gene result in Leber congenital amaurosis and cone-rod dystrophy-6 diseases. [provided by RefSeq].

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25, Flow cytometry: 1:25 (1x10e6 cells)

UniProt

Q02846

Host

Rabbit

Reactivity

Human, Rat

Immunogen

A portion of amino acids 540-570 from the human protein was used as the immunogen for the Retinal guanylyl cyclase 1 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P, FACS

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Retinal guanylyl cyclase 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Retinal guanylyl cyclase 1 antibody is available for research use only.

Storage Conditions

Aliquot the Retinal guanylyl cyclase 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Retinal guanylyl cyclase 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human thyroid tissue with Retinal guanylyl cyclase 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.