Rac GTPase-activating protein 1 Antibody / RACGAP1

Rho GTPases control a variety of cellular processes. There are 3 subtypes of Rho GTPases in the Ras superfamily of small G proteins: RHO, RAC, and CDC42. GTPase-activating proteins (GAPs) bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, Rho GAPs negatively regulate Rho-mediated signals. GAPs may also serve as effector molecules and play a role in signaling downstream of Rho and other Ras-like GTPases.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25, Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells)

UniProt

Q9H0H5

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 23-50 from the human protein was used as the immunogen for the Rac GTPase-activating protein 1 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P, IF, FACS

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Rac GTPase-activating protein 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Rac GTPase-activating protein 1 antibody is available for research use only.

Storage Conditions

Aliquot the Rac GTPase-activating protein 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Rac GTPase-activating protein 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear, cytoplasmic

Image Legend

IHC testing of FFPE human testis tissue with Rac GTPase-activating protein 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.