GAD2 Antibody / GAD65

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25, Immunofluorescence: 1:25

UniProt

Q05329

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 109-138 from the human protein was used as the immunogen for the GAD2 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P, IF

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the GAD2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This GAD2 antibody is available for research use only.

Storage Conditions

Aliquot the GAD2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the GAD2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.