Prolyl-tRNA synthetase Antibody / ProRS

Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis. Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25, Flow cytometry: 1:25 (1x10e6 cells)

UniProt

Q7L3T8

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 390-417 from the human protein was used as the immunogen for the Prolyl-tRNA synthetase antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P, FACS

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Prolyl-tRNA synthetase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Prolyl-tRNA synthetase antibody is available for research use only.

Storage Conditions

Aliquot the Prolyl-tRNA synthetase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Prolyl-tRNA synthetase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human kidney tissue with Prolyl-tRNA synthetase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.