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VPS52 Antibody / SACM2L

This gene encodes a protein that is similar to the yeast suppressor of actin mutations 2 gene. The yeast protein forms a subunit of the tetrameric Golgi-associated retrograde protein complex that is involved in vesicle trafficking from from both early and late endosomes, back to the trans-Golgi network. This gene is located on chromosome 6 in a head-to-head orientation with the gene encoding ribosomal protein S18.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000

UniProt

Q8N1B4

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 610-637 from the human protein was used as the immunogen for the VPS52 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

IF, FACS, IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the VPS52 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This VPS52 antibody is available for research use only.

Storage Conditions

Aliquot the VPS52 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the VPS52 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

Western blot testing of human MDA-MB-435 cell lysate with VPS52 antibody. Predicted molecular weight ~82 kDa (isoform 1) and 68 kDa (isoform 2) .

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