BCL10 Antibody

This gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25, Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000

UniProt

O95999

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 24-51 from the human protein was used as the immunogen for the BCL10 antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, IF, IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the BCL10 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This BCL10 antibody is available for research use only.

Storage Conditions

Aliquot the BCL10 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the BCL10 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, perinuclear

Image Legend

IHC testing of FFPE human tonsil tissue with BCL10 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.