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Alpha-amylase 2B Antibody / AMY2B

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000

UniProt

P19961

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 74-100 from the human protein was used as the immunogen for the Alpha-amylase 2B antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

IF, FACS, IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Alpha-amylase 2B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Alpha-amylase 2B antibody is available for research use only.

Storage Conditions

Aliquot the Alpha-amylase 2B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Alpha-amylase 2B antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

Western blot testing of human 1) HL60, 2) CEM and 3) K562 cell lysate with Alpha-amylase 2B antibody. Predicted molecular weight ~57 kDa.

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