CSF2 Antibody / Colony Stimulating Factor 2 / GMCSF

CSF2 is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq].

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000

UniProt

P04141

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 59-85 from the human protein was used as the immunogen for the CSF2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

IF, FACS, IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the CSF2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CSF2 antibody is available for research use only.

Storage Conditions

Aliquot the CSF2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the CSF2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

IHC testing of FFPE human lung carcinoma tissue with CSF2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.