IGF2BP1 Antibody / ZBP-1

IGF2BP1 is a member of the insulin-like growth factor 2 mRNA-binding protein family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, and regulating their translation. Two transcript variants encoding different isoforms have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25, Flow cytometry: 1:25 (1x10e6 cells)

UniProt

Q9NZI8

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 508-534 from the human protein was used as the immunogen for the IGF2BP1 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P, FACS

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the IGF2BP1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This IGF2BP1 antibody is available for research use only.

Storage Conditions

Aliquot the IGF2BP1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the IGF2BP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, nuclear

Image Legend

IHC testing of FFPE human testis tissue with IGF2BP1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.