ATG4A Antibody
Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25, Immunofluorescence: 1:25
UniProt
Q8WYN0
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for the ATG4A antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
1458CT808.66.25.69
Applications
WB, FACS, IHC-P, IF
Purity
Protein G affinity
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the ATG4A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This ATG4A antibody is available for research use only.
Storage Conditions
Aliquot the ATG4A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Location
Cytoplasmic
Image Legend
Western blot testing of human K562 cell lysate with ATG4A antibody. Predicted molecular weight ~45 kDa, commonly observed between 45-60 kDa.
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