ATG4A Antibody

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25, Immunofluorescence: 1:25

UniProt

Q8WYN0

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for the ATG4A antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

1458CT808.66.25.69

Applications

WB, FACS, IHC-P, IF

Purity

Protein G affinity

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the ATG4A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This ATG4A antibody is available for research use only.

Storage Conditions

Aliquot the ATG4A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the ATG4A antibody may be required due to differences in protocols and secondary/substrate sensitivity.