PSMA5 Antibody

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Immunofluorescence: 1:25, Immunohistochemistry (FFPE) : 1:25

UniProt

P28066

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human protein was used as the immunogen for the PSMA5 antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

426CT8.5.1

Applications

WB, IF, IHC-P

Purity

Protein G affinity

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the PSMA5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PSMA5 antibody is available for research use only.

Storage Conditions

Aliquot the PSMA5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the PSMA5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, nuclear

Image Legend

Western blot testing of human 1) liver, 2) HeLa, 3) HepG2, 4) MCF7, 5) mouse Neuro-2a and 6) rat PC-12 cell lysate with PSMA5 antibody. Predicted molecular weight ~26 kDa.