Neurofilament-66 Antibody / INA / Alpha Internexin

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene is a member of the intermediate filament family and is involved in the morphogenesis of neurons.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:25, Immunofluorescence: 1:25, Western blot: 1:500-1:2000

UniProt

Q16352

Host

Mouse

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 290-319 from the human protein was used as the immunogen for the Neurofilament-66 antibody.

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

257CT7.1.2

Applications

IHC-P, IF, WB

Purity

Protein G affinity

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Neurofilament-66 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Neurofilament-66 antibody is available for research use only.

Storage Conditions

Aliquot the Neurofilament-66 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Neurofilament-66 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

IHC testing of FFPE human brain tissue with Neurofilament-66 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.