MAP1LC3A antibody

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) . Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000

UniProt

Q9H492

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human protein was used as the immunogen for the MAP1LC3A antibody.

Clonality

Polyclonal

Isotype

Applications

IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the MAP1LC3A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This MAP1LC3A antibody is available for research use only.

Storage Conditions

Aliquot the MAP1LC3A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the MAP1LC3A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human heart tissue with MAP1LC3A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.