60 kDa Heat Shock Protein Antibody / Hsp60

Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25, Immunofluorescence: 1:25

UniProt

P10809

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human protein was used as the immunogen for the 60 kDa Heat Shock Protein antibody.

Clonality

Polyclonal

Isotype

Applications

WB, FACS, IHC-P, IF

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the 60 kDa Heat Shock Protein antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This 60 kDa Heat Shock Protein antibody is available for research use only.

Storage Conditions

Aliquot the 60 kDa Heat Shock Protein antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the 60 kDa Heat Shock Protein antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

Western blot testing of human 1) Jurkat, 2) HeLa, 3) HepG2, 4) MCF7, 5) mouse NIH 3T3 and 6) rat PC-12 cell lysate with 60 kDa Heat Shock Protein antibody. Predicted molecular weight ~60 kDa.