Vinculin Antibody
Actin filament (F-actin) -binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion (By similarity) .
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25
UniProt
Q64727
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant human protein was used as the immunogen for the Vinculin antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, FACS, IHC-P
Purity
Antigen affinity purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the Vinculin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Vinculin antibody is available for research use only.
Storage Conditions
Aliquot the Vinculin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Image Legend
Flow cytometry testing of fixed and permeabilized mouse NIH 3T3 cells with Vinculin antibody; Blue=isotype control, Green= Vinculin antibody.
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