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PGK1 Antibody

In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) .

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25

UniProt

P00558

Host

Mouse

Reactivity

Human, Mouse

Immunogen

Recombinant human protein was used as the immunogen for the PGK1 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

1086CT10.2.1

Applications

WB, FACS, IHC-P

Purity

Protein G affinity

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the PGK1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PGK1 antibody is available for research use only.

Storage Conditions

Aliquot the PGK1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the PGK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human kidney tissue with PGK1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

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