Density Regulated Protein Antibody / DENR

May be involved in the translation of target mRNAs by scanning and recognition of the initiation codon. Involved in translation initiation; promotes recruitmnet of aminoacetyled initiator tRNA to P site of 40S ribosomes. Can promote release of deacylated tRNA and mRNA from recycled 40S subunits following ABCE1-mediated dissociation of post-termination ribosomal complexes into subunits. Plays a role in the modulation of the translational profile of a subset of cancer-related mRNAs when recruited to the translational initiation complex by the oncogene MCTS1.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25, Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25

UniProt

O43583

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for the Density Regulated Protein antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

1542CT106.51.79

Applications

WB, IHC-P, FACS, IF

Purity

Protein G affinity

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Density Regulated Protein antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Density Regulated Protein antibody is available for research use only.

Storage Conditions

Aliquot the Density Regulated Protein antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Density Regulated Protein antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of human 1) A431, 2) skeletal muscle and 3) brain lysate with Density Regulated Protein antibody. Expected molecular weight ~22 kDa (unmodified), 27-31 kDa (modified) .