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BID Antibody / BH3-Interacting Domain Death Agonist

The major proteolytic product p15 BID allows the release of cytochrome c (By similarity) . Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25, Immunofluorescence: 1:25

UniProt

P55957

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for the BID antibody.

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

1519CT649.18.47

Applications

WB, IHC-P, IF

Purity

Protein G affinity

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the BID antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This BID antibody is available for research use only.

Storage Conditions

Aliquot the BID antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the BID antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human bladder tissue with BID antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

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