Histone H2AX Antibody / H2AFX

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000

UniProt

P16104

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 115-143 from the human protein was used as the immunogen for the Histone H2AX antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

938CT5.1.1

Applications

IHC-P, WB

Purity

Protein G affinity

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Histone H2AX antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Histone H2AX antibody is available for research use only.

Storage Conditions

Aliquot the Histone H2AX antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Histone H2AX antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear

Image Legend

IHC testing of FFPE human thymus tissue with Histone H2AX antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.