AIF Antibody / AIFM1 / Apoptosis-Inducing Factor
AIFM1 is a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, this protein induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000, Immunofluorescence: 1:25
UniProt
O95831
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 70-98 from the human protein was used as the immunogen for the AIF antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
FACS, IHC-P, WB, IF
Purity
Antigen affinity purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the AIF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This AIF antibody is available for research use only.
Storage Conditions
Aliquot the AIF antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Product Datasheet
https://www.nsjbio.com/tds-pdf/aif-antibody-aifm1-apoptosis-inducing-factor-f54468
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Location
Cytoplasmic, nuclear
Image Legend
IHC testing of FFPE human brain tissue with AIF antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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