SCNN1A Antibody

Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the alpha subunit, and mutations in this gene have been sociated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25, Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000

UniProt

P37088

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 365-391 from the human protein was used as the immunogen for the SCNN1A antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, IF, IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the SCNN1A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This SCNN1A antibody is available for research use only.

Storage Conditions

Aliquot the SCNN1A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the SCNN1A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, plasma membrane

Image Legend

IHC testing of FFPE human lung carcinoma tissue with SCNN1A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.