ATP5O Antibody

ATP5O is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. This protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25, Immunohistochemistry (FFPE) : 1:25

UniProt

P48047

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 38-64 from the human protein was used as the immunogen for the ATP5O antibody.

Clonality

Polyclonal

Isotype

Applications

WB, FACS, IF, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the ATP5O antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This ATP5O antibody is available for research use only.

Storage Conditions

Aliquot the ATP5O antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the ATP5O antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human skeletal muscle tissue with ATP5O antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.