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PEPCK1 Antibody / PCK1 / PEPC

This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. A mitochondrial isozyme of the encoded protein also has been characterized.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Immunofluorescence: 1:25, Immunohistochemistry (FFPE) : 1:25

UniProt

P35558

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A portion of amino acids 592-622 from the human protein was used as the immunogen for the PEPCK1 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, IF, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the PEPCK1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PEPCK1 antibody is available for research use only.

Storage Conditions

Aliquot the PEPCK1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the PEPCK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

Western blot testing of 1) human liver, 2) human kidney, 3) human NCI-H460, 4) mouse liver, 5) mouse kidney and 6) rat kidney lysate with PEPCK1 antibody. Predicted molecular weight: ~69 kDa.

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