HOMER3 Antibody

HOMER3 is a member of the HOMER family of postsynaptic density scaffolding proteins that share a similar domain structure consisting of an N-terminal Enabled/vasodilator-stimulated phosphoprotein homology 1 domain which mediates protein-protein interactions, and a carboxy-terminal coiled-coil domain and two leucine zipper motifs that are involved in self-oligomerization. This protein binds numerous other proteins including group I metabotropic glutamate receptors, inositol 1,4,5-trisphosphate receptors and amyloid precursor proteins and has been implicated in diverse biological functions such as neuronal signaling, T-cell activation and trafficking of amyloid beta peptides.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25

UniProt

Q9NSC5

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 281-308 from the human protein was used as the immunogen for the HOMER3 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, FACS, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the HOMER3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This HOMER3 antibody is available for research use only.

Storage Conditions

Aliquot the HOMER3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the HOMER3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, cell junction

Image Legend

IHC testing of FFPE human brain tissue with HOMER3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.