Aspartoacylase Antibody / ASPA

ASPA is an enzyme that catalyzes the conversion of N-acetyl_L-aspartic acid (NAA) to aspartate and acetate. NAA is abundant in the brain where hydrolysis by aspartoacylase is thought to help maintain white matter. This protein is an NAA scavenger in other tissues.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:25, Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25

UniProt

P45381

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 82-110 from the human protein was used as the immunogen for the Aspartoacylase antibody.

Clonality

Polyclonal

Isotype

Applications

IF, WB, FACS, IHC-P

Purity

SAS precipitation

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Aspartoacylase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Aspartoacylase antibody is available for research use only.

Storage Conditions

Aliquot the Aspartoacylase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Aspartoacylase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear, cytoplasmic

Image Legend

IHC testing of FFPE human skeletal muscle tissue with Aspartoacylase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.