CDK14 Antibody / Cyclin-dependent kinase 14 / PFTAIRE-1

Serine/threonine-protein kinase involved in the control of the eukaryotic cell cycle, whose activity is controlled by an associated cyclin. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by mediating the phosphorylation of LRP6 at 'Ser-1490', leading to the activation of the Wnt signaling pathway. Acts as a regulator of cell cycle progression and cell proliferation via its interaction with CCDN3. Phosphorylates RB1 in vitro, however the relevance of such result remains to be confirmed in vivo. May also play a role in meiosis, neuron differentiation and may indirectly act as a negative regulator of insulin-responsive glucose transport. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25

UniProt

O94921

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 67-96 from the human protein was used as the immunogen for the CDK14 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, FACS, IHC-P

Purity

SAS precipitation

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the CDK14 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CDK14 antibody is available for research use only.

Storage Conditions

Aliquot the CDK14 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the CDK14 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear, cytoplasmic

Image Legend

IHC testing of FFPE human testis tissue with CDK14 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.