ZWINT Antibody

ZWINT is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25

UniProt

O95229

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 59-88 from the human protein was used as the immunogen for the ZWINT antibody.

Clonality

Polyclonal

Isotype

Applications

WB, FACS, IHC-P

Purity

SAS precipitation

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the ZWINT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This ZWINT antibody is available for research use only.

Storage Conditions

Aliquot the ZWINT antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the ZWINT antibody may be required due to differences in protocols and secondary/substrate sensitivity.