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HPX Antibody / Hemopexin

HPX is a plasma glycoprotein that binds heme with high affinity. The protein is an acute phase protein that transports heme from the plasma to the liver and may be involved in protecting cells from oxidative stress.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells)

UniProt

P02790

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A portion of amino acids 200-227 from the human protein was used as the immunogen for the HPX antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

IHC-P, WB, FACS

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the HPX antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This HPX antibody is available for research use only.

Storage Conditions

Aliquot the HPX antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the HPX antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

IHC testing of FFPE human liver tissue with HPX antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.

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