Pro-MCH Antibody / PMCH

The melanin-concentrating hormone (MCH) is a cyclic neuropeptide isolated initially from salmon pituitary gland and later from rat hypothalamus (summarized by Nahon et al., 1992 [PubMed 1572663]) . In mammals, MCH perikarya are confined largely to the lateral hypothalamus and zona incerta area with extensive neuronal projections throughout the brain, including the neurohypophysis. The anatomic distribution suggests a neurotransmitter or neuromodulator role for MCH in a broad array of neuronal functions directed toward the regulation of goal-directed behavior, such as food intake, and general arousal. MCH and 2 other putative neuropeptides, NEI and NGE, are encoded by the same precursor and appear colocalized in nerve cells and in many instances within the projections. The precursor is designated pro-melanin-concentrating hormone (PMCH) .

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25

UniProt

P20382

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 94-122 from the human protein was used as the immunogen for the Pro-MCH antibody.

Clonality

Polyclonal

Isotype

Applications

WB, FACS, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Pro-MCH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Pro-MCH antibody is available for research use only.

Storage Conditions

Aliquot the Pro-MCH antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Pro-MCH antibody may be required due to differences in protocols and secondary/substrate sensitivity.