TENT2 Antibody / GLD2

GLD2 is cytoplasmic poly (A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly (A) tail. In contrast to the canonical nuclear poly (A) RNA polymerase, it only adds poly (A) to selected cytoplasmic mRNAs. GLD2 does not play a role in replication-dependent histone mRNA degradation.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:25, Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000

UniProt

Q6PIY7

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 58-87 from the human protein was used as the immunogen for the TENT2 antibody.

Clonality

Polyclonal

Isotype

Applications

IF, IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the TENT2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This TENT2 antibody is available for research use only.

Storage Conditions

Aliquot the TENT2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the TENT2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear, cytoplasmic

Image Legend

IHC testing of FFPE human cerebellum tissue with TENT2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.