Phospho-CREB Antibody (pS133)

CREB is a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, (CRE), a sequence present in many viral and cellular promoters. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000

UniProt

P16220

Host

Rabbit

Reactivity

Human

Immunogen

The amino acids surrounding phosphorylated serine 133 from the human protein were used as the immunogen for the phospho-CREB antibody.

Clonality

Polyclonal

Isotype

Applications

IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the phospho-CREB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This phospho-CREB antibody is available for research use only.

Storage Conditions

Aliquot the phospho-CREB antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the phospho-CREB antibody may be required due to differences in protocols and secondary/substrate sensitivity.