YWHAG Antibody / 14-3-3 gamma

YWHAG belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25

UniProt

P61981

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 136-164 from the human protein was used as the immunogen for the YWHAG antibody.

Clonality

Polyclonal

Isotype

Applications

WB, FACS, IHC-P

Purity

SAS precipitation

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the YWHAG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This YWHAG antibody is available for research use only.

Storage Conditions

Aliquot the YWHAG antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the YWHAG antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human colon carcinoma tissue with YWHAG antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.