CDKN1A Antibody / p21
May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin- dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D- CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1:25, Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25
UniProt
P38936
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 133-164 from the human protein was used as the immunogen for the CDKN1A antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IF, WB, IHC-P
Purity
Antigen affinity purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the CDKN1A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This CDKN1A antibody is available for research use only.
Storage Conditions
Aliquot the CDKN1A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Location
Nuclear
Image Legend
Western blot testing of human 1) HeLa, 2) HUVEC, 3) MCF7, 4) SH-SY5Y and 5) mouse lung lysate with CDKN1A antibody.
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