Cytochrome C1 Antibody / CYC1

This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:25, Immunofluorescence: 1:25, Western blot: 1:500-1:2000

UniProt

P08574

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 142-176 from the human protein was used as the immunogen for the Cytochrome C1 antibody.

Clonality

Polyclonal

Isotype

Applications

IHC-P, IF, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Cytochrome C1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Cytochrome C1 antibody is available for research use only.

Storage Conditions

Aliquot the Cytochrome C1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Cytochrome C1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

Western blot testing of human 1) A431, 2) HeLa, 3) HepG2, 4) MCF7, 5) U-251 MG and 6) mouse brain lysate with Cytochrome C1 antibody. Predicted molecular weight ~35 kDa.