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HBE1 Antibody / Hemoglobin subunit epsilon

The epsilon globin gene (HBE) is normally expressed in the embryonic yolk sac: two epsilon chains together with two zeta chains (an alpha-like globin) constitute the embryonic hemoglobin Hb Gower I; two epsilon chains together with two alpha chains form the embryonic Hb Gower II. Both of these embryonic hemoglobins are normally supplanted by fetal, and later, adult hemoglobin. The five beta-like globin genes are found within a 45 kb cluster on chromosome 11 in the following order: 5'-epsilon - G-gamma - A-gamma - delta - beta-3'

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25

UniProt

P02100

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 55-83 from the human protein was used as the immunogen for the HBE1 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, FACS, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the HBE1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This HBE1 antibody is available for research use only.

Storage Conditions

Aliquot the HBE1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the HBE1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, secreted

Image Legend

Western blot testing of human 1) placenta and 2) K562 lysate with HBE1 antibody. Predicted molecular weight ~16 kDa.

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