Exportin 2 Antibody / CSE1L

Proteins that carry a nuclear localization signal (NLS) are transported into the nucleus by the importin-alpha/beta heterodimer. Importin-alpha binds the NLS, while importin-beta mediates translocation through the nuclear pore complex. After translocation, RanGTP binds importin-beta and displaces importin-alpha. Importin-alpha must then be returned to the cytoplasm, leaving the NLS protein behind. CSE1L binds strongly to NLS-free importin-alpha, and this binding is released in the cytoplasm by the combined action of RANBP1 and RANGAP1. In addition, CSE1L may play a role both in apoptosis and in cell proliferation.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:25, Immunofluorescence: 1:25, Western blot: 1:500-1:2000

UniProt

P55060

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 55-84 from the human protein was used as the immunogen for the Exportin 2 antibody.

Clonality

Polyclonal

Isotype

Applications

IHC-P, IF, WB

Purity

SAS precipitation

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Exportin 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Exportin 2 antibody is available for research use only.

Storage Conditions

Aliquot the Exportin 2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Exportin 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear, cytoplasmic

Image Legend

Western blot testing of human 1) HeLa, 2) Jurkat, 3) Ramos and 4) mouse NIH 3T3 cell lysate with Exportin 2 antibody. Predicted molecular weight ~110 kDa.