POLG Antibody
Mitochondrial DNA polymerase is heterotrimeric, consisting of a homodimer of accessory subunits plus a catalytic subunit. The protein encoded by this gene is the catalytic subunit of mitochondrial DNA polymerase. The encoded protein contains a polyglutamine tract near its N-terminus that may be polymorphic. Defects in this gene are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO), Alpers-Huttenlocher syndrome (AHS), and mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE) . Two transcript variants encoding the same protein have been found for this gene.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells), Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25
UniProt
P54098
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 1120-1148 from the human protein was used as the immunogen for the POLG antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
IF, FACS, WB, IHC-P
Purity
Antigen affinity purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the POLG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This POLG antibody is available for research use only.
Storage Conditions
Aliquot the POLG antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Product Datasheet
https://www.nsjbio.com/tds-pdf/polg-antibody-f54379
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Location
Cytoplasmic
Image Legend
Western blot testing of human HepG2 cell lysate with POLG antibody. Predicted molecular weight ~140 kDa.
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