POLG Antibody

Mitochondrial DNA polymerase is heterotrimeric, consisting of a homodimer of accessory subunits plus a catalytic subunit. The protein encoded by this gene is the catalytic subunit of mitochondrial DNA polymerase. The encoded protein contains a polyglutamine tract near its N-terminus that may be polymorphic. Defects in this gene are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO), Alpers-Huttenlocher syndrome (AHS), and mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE) . Two transcript variants encoding the same protein have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells), Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25

UniProt

P54098

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 1120-1148 from the human protein was used as the immunogen for the POLG antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

IF, FACS, WB, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the POLG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This POLG antibody is available for research use only.

Storage Conditions

Aliquot the POLG antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the POLG antibody may be required due to differences in protocols and secondary/substrate sensitivity.