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TCF3 Antibody / Transcription factor E2-alpha

The TCF3 gene, also called E2A, encodes 2 basic helix-loop-helix (bHLH) transcription factors, E12 and E47, through alternative splicing. E12 and E47 are involved in regulation of immunoglobulin gene expression.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25

UniProt

P15923

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 506-534 from the human protein was used as the immunogen for the TCF3 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, FACS, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the TCF3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This TCF3 antibody is available for research use only.

Storage Conditions

Aliquot the TCF3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the TCF3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

IHC testing of FFPE human tonsil tissue with TCF3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

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