RAR alpha Antibody / RARA

This gene represents a nuclear retinoic acid receptor. The encoded protein, retinoic acid receptor alpha, regulates transcription in a ligand-dependent manner. This gene has been implicated in regulation of development, differentiation, apoptosis, granulopoeisis, and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia. Alternatively spliced transcript variants have been found for this locus.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25, Immunofluorescence: 1:25

UniProt

P10276

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 322-349 from the human protein were used as the immunogen for the RAR alpha antibody.

Clonality

Polyclonal

Isotype

Applications

WB, FACS, IHC-P, IF

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the RAR alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This RAR alpha antibody is available for research use only.

Storage Conditions

Aliquot the RAR alpha antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the RAR alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

IHC testing of FFPE mouse cerebellum tissue with RAR alpha antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.