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SFRP5 Antibody

Secreted frizzled-related protein 5 (SFRP5) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas. [provided by RefSeq].

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25

UniProt

Q5T4F7

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 190-219 from the human protein were used as the immunogen for the SFRP5 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the SFRP5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This SFRP5 antibody is available for research use only.

Storage Conditions

Aliquot the SFRP5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the SFRP5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

IHC testing of FFPE human pancreas tissue with SFRP5 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.

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