Acid Sphingomyelinase Antibody / SMPD1

The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB) . Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq].

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells)

UniProt

P17405

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 391-419 from the human protein was used as the immunogen for the Acid Sphingomyelinase antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

IHC-P, WB, FACS

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Acid Sphingomyelinase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Acid Sphingomyelinase antibody is available for research use only.

Storage Conditions

Aliquot the Acid Sphingomyelinase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Acid Sphingomyelinase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of human 1) K562, 2) MCF7, 3) HepG2 and 4) A431 cell lysate with Acid Sphingomyelinase antibody. Predicted molecular weight ~70 kDa.